Hoechst 33342 solution
NettetDAPI染色液 (DAPI Staining Solution)是经过精心优化几乎适用于所有常见细胞和组织细胞核染色的染色液。. DAPI，即2- (4-Amidinophenyl)-6-indolecarbamidine dihydrochloride，也称DAPI dihydrochloride，分子式为C 16 H 15 N 5 ·2HCl ，分子量为350.25 ，CAS Number 28718-90-3。. DAPI是一种可以穿透细胞 ... NettetBio-Rad's PureBlu™ Hoechst 33342 Dye is ideal for routine nuclear staining in fluorescence microscopy, cell imaging applications and cell cycle analysis in flow cytometry. It is available as a ready-to-reconstitute, highly pure powder form, with only one dilution step required to obtain a ready-to-use solution. Hoechst 33342 Binding
Hoechst 33342 solution
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NettetHoechst 33342 nucleic acid stain is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA. This dye is often used to distinguish … NettetThe Hoechst stains may be used on live or fixed cells, and are often used as a substitute for another nucleic acid stain, DAPI. The key difference between them is that the …
NettetFeatures of Hoechst 33342 Fluorescent Stain: • Hoechst dye—blue fluorescent stain specific for DNA (i.e., nuclei of eukaryotic cells) • Convenient—provided as an easy-to … Features of Hoechst 33342 Fluorescent Stain: • Hoechst dye—blue fluorescent st… TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes These dyes can also stain Gram-positive and Gram-negative bacteria. There are … The Thermo Scientific FITC Antibody Labeling Kit is a multi-component kit contai… TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes NettetIncubate the cells in the Hoechst labeling solution (from Step 1) for 10-30 min at room temperature. 4. Aspirate the labeling solution. Rinse the cells three times in PBS +. 5. Mount the coverslips as described in Mounting Live Cells onto Microscope Slides (Chazotte 2011b). 6. Image the cells (λ ex ~353 nm, λ em ~483 nm for Hoechst 33342).
NettetThe BD Pharmingen™ Hoechst 33342 Solution is a reagent for the fluorescent staining of DNA and nuclei in live or fixed cells. Hoechst 33342 is a bisbenzimidazole dye with … NettetHoechst 33342 solution, 1 mg/ml in water - CAS-Number 23491-52-3 - Order from Chemodex Inquiry Hoechst 33342 solution, 1 mg/ml in water SKU B0447 Category: Fluorescent Detection Synonyms 2'- (4-Ethoxyphenyl)-5- (4-methyl-1-piperazinyl)-2,5'-bi-1H-benzimidazole trihydrochloride , BisBenzimide H 33342 , HOE 33342 23491-52-3 …
NettetHoechst dyes are cell membrane-permeant, minor groove-binding blue fluorescent DNA stains. These dyes are widely used in cell cycle and apoptosis studies as nuclear counterstains. Biotium offers Hoechst 33258 and Hoechst 33342 dyes …
Nettet28. feb. 2024 · Hoechst 33342 staining also showed obvious morphological characteristics of apoptosis in T24 cells incubated with Au–Ag@PDA NPs at 3 and 24 hours after laser irradiation. In particular, the apoptotic cells stained with Hoechst 33342 presented brighter blue fluorescence due to karyopyknosis and chromatin condensation in the … physiotherapie mobili hannoverNettetHoechst 33342 is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA. This dye is often used to distinguish condensed … too stiff arrow spineNettetCellstain Hoechst 33342 溶液 (1mg/ml H2O) Cellstain Hoechst 33342 Solution (1mg/ml H2O) 製造元 : (株)同仁化学研究所 保存条件 : 冷蔵 (室温輸送) CAS RN ® : 23491-52-3 … too stimulativeNettet18. jun. 2024 · Subsequently, the cells are incubated in a solution of 2 µM Hoechst 33342 or 3 µM DAPI in 20 mM Tris-HCl, pH 7, 150 mM NaCl for 30 minutes on a laboratory shaker (100 µl per well of a 96-well ... too stoned bumpin uglies lyricsNettet27. mar. 2015 · DNA condensation in cells was stained with Hoechst 33342 solution and visualized using a fluorescent microscope (magnification 100×). BSA, bovine serum albumin; PA, palmitate; Vc, Vitamin C. Figure 3. Protective effect of hispidin on palmitate-induced oxidative stress in C2C12 myotubes. physiotherapie mönchengladbachNettetProduct number: 910-3015 Size: 1 ml Storage: 2 – 7°C Solution 15 – Hoechst 33342 is a staining reagent containing Hoechst 33342, which stains the nuclei of living, fixed and permeabilized cells. Solution 15 – Hoechst 33342 is a cell permeable dye that preferably stains doublestranded DNA. physiotherapie moers st josefphysiotherapie mondorf