Rice chip-seq
Webb12 apr. 2024 · Heat stress (HS) caused by high-temperature weather seriously threatens international food security. Indeed, as an important food crop in the world, the yield and quality of rice are frequently affected by HS. Therefore, clarifying the molecular mechanism of heat tolerance and cultivating heat-tolerant rice varieties is urgent. Here, we … Webb23 aug. 2024 · Therefore, we searched previously published ChIP-seq data of IPA1 33, and noticed that seven GA-related genes, including EUI1, GA2ox6, SLR1, GSR1 (GAST family …
Rice chip-seq
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WebbChIP-chip technology refers to the utilization of a DNA microarray chip to analyze ChIP-immune enriched DNA fragments. Using genome tiling microarray technology allows for a whole-genome analysis of proteins that are bound to isolated DNA and generates a high resolution genomic map of protein-binding and protein modifications. Webb23 mars 2024 · The AA allele was mainly found in indica rice, the TT allele was mainly found in japonica rice (Figure 4c), and the AT allele was present in both cultivars (Figure 4c). Geographical localization analysis revealed that varieties with the AA allele were primarily distributed in tropical regions, whereas varieties with the TT allele were …
WebbChromatin immunoprecipitation combined with high-throughput sequencing (ChIP-seq) can be used to map binding sites of a protein of interest in the genome. Histone modifications usually occupy broad chromatin domains and result in diffuse patterns in ChIP-seq data that make it difficult to identify signal enrichment. WebbRice feeds half the world’s population, ... (ChIP)-seq experiment using GST antibodies against GST-tagged BSR-D1 protein. We identified a list of 14 genes . Topping the list of genes pulled down by the BSR-D1 protein are two …
Webb13 juli 2015 · To gain a better understanding of the role that the circadian clock plays in the control of these multiple biological processes through CCA1, either directly or through … WebbThis database contains 972 datasets, which is the largest one for rice epigenomics up to our best knowledge, which can help us to outline the rice epigenome in a comprehensive way, and provide...
Webb30 juni 2014 · ChIP assay followed by next generation sequencing (ChIP-seq) or microarray (ChIP-chip) is often used to study patterns of protein-binding profiles in …
WebbRNA-Seq has been applied to sequence the transcriptomes of several crop plants including, soybean, rice, and grapes (Lu et al., 2010; Severin et al., ... External CLIP-seq and Pol II ChIP-seq datasets were downloaded in raw format from the Gene Expression Omnibus (https: ... rp tech hyderabadWebbfor the ChIP-seq analysis in Arabidopsis because of its high e’cient transformation system. For the plant species that are recalcitrant to transformation, the speci#c monoclonal or polyclonal antibodies are o&en used for ChIP-seq (Table 10.1). Illumina GA II or HiSeq X is the mostly used deep sequencing platform for ChIP-seq (Table rp the crew 2Webb9 sep. 2024 · For example, the RIS 705 may modulate (e.g., scramble) at least one of the phase, the amplitude, the frequency, or the polarization of the SSB signal (for each SSB redirected by the RIS 705) at the symbol boundaries of the SSB signal, in accordance with an RIS-specific pattern or sequence of modulation identified in the modulation signature … rp thermometer\\u0027sWebbLow cell number ChIP-seq reveals chromatin state-based regulation of gene transcription in the rice male meiocytes Low cell number ChIP-seq reveals chromatin state-based … rp thermostat\\u0027sWebb15 maj 2024 · Transcription factors–DNA interactions in rice: identification and verification Zijie Shen, Zijie Shen Institute of Fundamental and Frontier Sciences , University of … rp theWebbPeak Calling. Peak calling, the next step in our workflow, is a computational method used to identify areas in the genome that have been enriched with aligned reads as a consequence of performing a ChIP-sequencing experiment. For ChIP-seq experiments, what we observe from the alignment files is a strand asymmetry with read densities on … rp thermometer\u0027sWebb9 nov. 2024 · This protocol provides specific details of how ChIP can be performed on cells. The output DNA produced using this protocol can be analyzed using either qPCR … rp they\\u0027ve